Immunological laboratory methods to detect HIV+ samples from different patients

Event Description:

Date of the Event:14th Nov 2017
Event Conduction Duration:09.30 AM to 12:30 PM
Event Venue:ART center and ICTC's Laboratory, Civil Hospital, Nashik
Event Resource Person Details (Speaker Image, Speaker name, Designation, company name):
1)Mr. Mahesh Loharkar,
Site Incharge
STP Tapovan Nasik
Name of Event Coordinator with contact details:): Dr. Ruchi Lakra (9650664973
Dr Vinod Sutoane (9890976863)
Outline of Program

After AIDS awareness lecture, Hands on training for student regarding detection methods used in field was conducted within civil hospital. Technician at civil hospital explained the concept and procedure for 3 different quick methods used in field. Hands on training for students were also performed with their own samples for HIV infection with confidential results.
Objective of Program:

The biology of HIV virus has been explained during theory classes. The immunological responses generated by HIV virus after infection, further its consequences on immune system was taught by Dr. Ruchi Lakra during scheduled classroom lecture. However, to understand practical approach to detect infection has been performed at civil hospital under the supervision of Dr. Ruchi Lakra (Assistant Professor, Sandip University) and Ms. Hemangi Joshi (Technician, Civil hospital). Experimental demonstration of samples available from different patient (confidential) sample was performed.


Outcome of Program:

Sandip University believes in practical approach of learning of science subjects, hence such laboratory visits are conducted to make student understand and apply the concepts they have learned during classroom session. Knowledge of various aspects in ICTC and ART center was also provided by Mrs. Hemangi Joshi and Mrs Ashwani Bhosale. Students learned following points during this lab training session conducted at Civil hospital:

1. Students understood the precaution taken while handling blood samples of various types of patients.

2. Students come know about processing blood sample and various methods to detect HIV infection from whole blood, Serum and Plasma

3. Preservation of chemical and kits at appropriate place and temperature is important to maintain their life shelf, especially in cases of detection of human disease samples.

4. Hands on experience on the Immunological practical approach to detect the HIV infection in blood samples.

5. Immunological mechanism behind functioning of kit, interaction among antigen and antibody to precipitated and result into colored precipitate.

6. Detection of HIV infection in newly born child is different. Blood sample from infants are taken from to and blood samples are processed further for Polymerase Chain Reaction (PCR).

7. Importance of testing in prenuptial couples and pregnant women

8. Reports of HIV patient should be confidential, revealing its status is unlawful act. It is violation of Human right and punishable offense.

9. They learned to proper ways to dispose bio-hazardous waste from human blood samples and other biological wastes.

10. Importance of counseling before and after testing of patients.

11. Regular medicine at ART center can improve quality of life for HIV+ patient and delay the adverse AIDS condition.


All together the holistic view towards the whole process was presented to student to understand the urgency for awareness programme.

Event Date

14 November 2017 - 14 November 2017